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pxpr brd003 genetic reagent  (Addgene inc)


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    Addgene inc pxpr brd003 genetic reagent
    Pxpr Brd003 Genetic Reagent, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pxpr+brd003+genetic+reagent/pXPR_BRD003+PSMB5+guide+2+(Plasmid+%23117074)/10__7554_slash_elife__44161-349-74-84
    Average 90 stars, based on 1 article reviews
    pxpr brd003 genetic reagent - by Bioz Stars, 2026-09
    90/100 stars

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    shRNA:

    Article Title: Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.44161 17 of 32 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) UBE2C NA Strain, strain background (Mus musculus) CrTac:NCr-Foxn1nu Taconic Biosciences RRID:IMSR_TAC:ncrnu Genetic reagentLuciferase this paper RRID:Addgene_117072 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) UBE2C guide 1 this paper RRID:Addgene_117068 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) UBE2C guide 2 this paper RRID:Addgene_117071 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) PSMB5 guide 1 this paper RRID:Addgene_117073 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) PSMB5 guide 2 this paper RRID:Addgene_117074 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) pDONR223 SMARCB1 this paper RRID:Addgene_111181 Genetic reagent (Homo sapiens) pLXI_401 LacZ this paper RRID:Addgene_111183 Backbone: pLXI_403 Genetic reagent (Homo sapiens) pLXI_403 LacZ this paper RRID:Addgene_111184 Backbone: pLXI_403 Genetic reagent (Homo sapiens) pLXI_401 SMARCB1 this paper RRID:Addgene_111182 Backbone: pLXI_401 Genetic reagent (Homo sapiens) pLXI_403 SMARCB1 this paper RRID:Addgene_111185 Backbone: pLXI_403 Genetic reagent (Homo sapiens) CP1050 PMID: 27329820 Druggable Cancer Targets v1.0 library (shRNA) Genetic reagent (Homo sapiens) CP1074 PMID: 27329820 Druggable Cancer Targets v1.0 library (CRISPR-Cas9) Cell line (Homo sapiens) CLF_PEDS0005_N this paper normal kidney cell line Cell line (Homo sapiens) CLF_PEDS0005_T1 this paper primary RMC cell line Cell line (Homo sapiens) CLF_PEDS0005_T2A this paper metastatic RMC cell line (adherent) Cell line (Homo sapiens) CLF_PEDS0005_T2B this paper metastatic RMC cell line (suspension) Cell line (Homo sapiens) CLF_PEDS9001_T1 this paper primary RMC cell line Cell line (Homo sapiens) CLF_PEDS1012_T1 this paper Wilms tumor cell line Cell line (Homo sapiens) G-401 ATCC CRL-1441 RRID:CVCL_0270 Cell line (Homo sapiens) RPMI 8226 ATCC CCL-155 RRID:CVCL_0014 Cell line (Homo sapiens) NCI-H2172 ATCC CRL-5930 RRID:CVCL_1537 Cell line (Homo sapiens) A549 ATCC CCL-185 RRID:CVCL_0023 Cell line (Homo sapiens) HA1E other Cell line maintained in W. C. Hahn lab Cell line (Homo sapiens) JMSU1 PMID: 30777879 RRID:CVCL_2081 Antibody anti-cytokeratin CAM5.2 BD Biosciences 349205 RRID:AB_2134314 Immunohistochemistry Continued on next page Hong et al. eLife 2019;8:e44161. .. DOI: https://doi.org/10.7554/eLife.44161 19 of 32 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Rneasy Plus Mini Kit Qiagen 74134 Commercial assay or kit Qubit RNA HS Assay Kit Thermo Fisher Scientific Q32852 Commercial assay or kit KAPA Stranded mRNA-Seq Kit Kapa Biosystems KK8420 Commercial assay or kit KAPA Library Quantification Kit Kapa Biosystems KK4835 Commercial assay or kit High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific 4368814 Commercial assay or kit Power SYBR Green PCR Master Mix Thermo Fisher Scientific 4368708 Commercial assay or kit Senescence b-Galactosidase Staining Kit Cell Signaling Technology 9860S Commercial assay or kit CellTiter-Glo Luminescent Cell Viability Assay Promega G7570 Commercial assay or kit Proteasome-Glo Chymotrypsin-Like Cell-based Assay Promega G8660 Commercial assay or kit Annexin V: FITC Apoptosis Detection Kit Thermo Fisher Scientific BD 556547 Commercial assay or kit PI/Rnase Staining Buffer BD Pharmingen 550825 Commercial assay or kit FxCycle PI/Rnase Staining Solution Invitrogen F10797 Chemical compound, drug bortezomib (PS-341) Selleck Chemicals S1013 in vitro studies Chemical compound, drug ixazomib (MLN2238) Selleck Chemicals S2180 in vitro studies Chemical compound, drug nocodazole Selleck Chemicals S2775 in vitro studies Chemical compound, drug Ro-3306 Selleck Chemicals S7747 in vitro studies Chemical compound, drug MLN2238 MedChem Express HY-10453 in vivo studies Software, algorithm SvABA v0.2.1 PMID: 29535149 Software, algorithm FlowJo v10.0 RRID:SCR_008520 Software, algorithm ComBat RRID:SCR_010974 Software, algorithm GraphPad Prism v8.0 RRID:SCR_002798 Software, algorithm GSEA Broad Institute RRID:SCR_003199 Software, algorithm GATK v.4.0.4.0 PMID: 20644199 RRID:SCR_001876 Software, algorithm TopHat v2.0.11 PMID: 22383036 RRID:SCR_013035 Software, algorithm DESeq2 PMID: 25516281 RRID:SCR_015687

    CRISPR:

    Article Title: Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.44161 17 of 32 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) UBE2C NA Strain, strain background (Mus musculus) CrTac:NCr-Foxn1nu Taconic Biosciences RRID:IMSR_TAC:ncrnu Genetic reagentLuciferase this paper RRID:Addgene_117072 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) UBE2C guide 1 this paper RRID:Addgene_117068 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) UBE2C guide 2 this paper RRID:Addgene_117071 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) PSMB5 guide 1 this paper RRID:Addgene_117073 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) PSMB5 guide 2 this paper RRID:Addgene_117074 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) pDONR223 SMARCB1 this paper RRID:Addgene_111181 Genetic reagent (Homo sapiens) pLXI_401 LacZ this paper RRID:Addgene_111183 Backbone: pLXI_403 Genetic reagent (Homo sapiens) pLXI_403 LacZ this paper RRID:Addgene_111184 Backbone: pLXI_403 Genetic reagent (Homo sapiens) pLXI_401 SMARCB1 this paper RRID:Addgene_111182 Backbone: pLXI_401 Genetic reagent (Homo sapiens) pLXI_403 SMARCB1 this paper RRID:Addgene_111185 Backbone: pLXI_403 Genetic reagent (Homo sapiens) CP1050 PMID: 27329820 Druggable Cancer Targets v1.0 library (shRNA) Genetic reagent (Homo sapiens) CP1074 PMID: 27329820 Druggable Cancer Targets v1.0 library (CRISPR-Cas9) Cell line (Homo sapiens) CLF_PEDS0005_N this paper normal kidney cell line Cell line (Homo sapiens) CLF_PEDS0005_T1 this paper primary RMC cell line Cell line (Homo sapiens) CLF_PEDS0005_T2A this paper metastatic RMC cell line (adherent) Cell line (Homo sapiens) CLF_PEDS0005_T2B this paper metastatic RMC cell line (suspension) Cell line (Homo sapiens) CLF_PEDS9001_T1 this paper primary RMC cell line Cell line (Homo sapiens) CLF_PEDS1012_T1 this paper Wilms tumor cell line Cell line (Homo sapiens) G-401 ATCC CRL-1441 RRID:CVCL_0270 Cell line (Homo sapiens) RPMI 8226 ATCC CCL-155 RRID:CVCL_0014 Cell line (Homo sapiens) NCI-H2172 ATCC CRL-5930 RRID:CVCL_1537 Cell line (Homo sapiens) A549 ATCC CCL-185 RRID:CVCL_0023 Cell line (Homo sapiens) HA1E other Cell line maintained in W. C. Hahn lab Cell line (Homo sapiens) JMSU1 PMID: 30777879 RRID:CVCL_2081 Antibody anti-cytokeratin CAM5.2 BD Biosciences 349205 RRID:AB_2134314 Immunohistochemistry Continued on next page Hong et al. eLife 2019;8:e44161. .. DOI: https://doi.org/10.7554/eLife.44161 19 of 32 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Rneasy Plus Mini Kit Qiagen 74134 Commercial assay or kit Qubit RNA HS Assay Kit Thermo Fisher Scientific Q32852 Commercial assay or kit KAPA Stranded mRNA-Seq Kit Kapa Biosystems KK8420 Commercial assay or kit KAPA Library Quantification Kit Kapa Biosystems KK4835 Commercial assay or kit High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific 4368814 Commercial assay or kit Power SYBR Green PCR Master Mix Thermo Fisher Scientific 4368708 Commercial assay or kit Senescence b-Galactosidase Staining Kit Cell Signaling Technology 9860S Commercial assay or kit CellTiter-Glo Luminescent Cell Viability Assay Promega G7570 Commercial assay or kit Proteasome-Glo Chymotrypsin-Like Cell-based Assay Promega G8660 Commercial assay or kit Annexin V: FITC Apoptosis Detection Kit Thermo Fisher Scientific BD 556547 Commercial assay or kit PI/Rnase Staining Buffer BD Pharmingen 550825 Commercial assay or kit FxCycle PI/Rnase Staining Solution Invitrogen F10797 Chemical compound, drug bortezomib (PS-341) Selleck Chemicals S1013 in vitro studies Chemical compound, drug ixazomib (MLN2238) Selleck Chemicals S2180 in vitro studies Chemical compound, drug nocodazole Selleck Chemicals S2775 in vitro studies Chemical compound, drug Ro-3306 Selleck Chemicals S7747 in vitro studies Chemical compound, drug MLN2238 MedChem Express HY-10453 in vivo studies Software, algorithm SvABA v0.2.1 PMID: 29535149 Software, algorithm FlowJo v10.0 RRID:SCR_008520 Software, algorithm ComBat RRID:SCR_010974 Software, algorithm GraphPad Prism v8.0 RRID:SCR_002798 Software, algorithm GSEA Broad Institute RRID:SCR_003199 Software, algorithm GATK v.4.0.4.0 PMID: 20644199 RRID:SCR_001876 Software, algorithm TopHat v2.0.11 PMID: 22383036 RRID:SCR_013035 Software, algorithm DESeq2 PMID: 25516281 RRID:SCR_015687

    Suspension:

    Article Title: Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.44161 17 of 32 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) UBE2C NA Strain, strain background (Mus musculus) CrTac:NCr-Foxn1nu Taconic Biosciences RRID:IMSR_TAC:ncrnu Genetic reagentLuciferase this paper RRID:Addgene_117072 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) UBE2C guide 1 this paper RRID:Addgene_117068 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) UBE2C guide 2 this paper RRID:Addgene_117071 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) PSMB5 guide 1 this paper RRID:Addgene_117073 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) PSMB5 guide 2 this paper RRID:Addgene_117074 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) pDONR223 SMARCB1 this paper RRID:Addgene_111181 Genetic reagent (Homo sapiens) pLXI_401 LacZ this paper RRID:Addgene_111183 Backbone: pLXI_403 Genetic reagent (Homo sapiens) pLXI_403 LacZ this paper RRID:Addgene_111184 Backbone: pLXI_403 Genetic reagent (Homo sapiens) pLXI_401 SMARCB1 this paper RRID:Addgene_111182 Backbone: pLXI_401 Genetic reagent (Homo sapiens) pLXI_403 SMARCB1 this paper RRID:Addgene_111185 Backbone: pLXI_403 Genetic reagent (Homo sapiens) CP1050 PMID: 27329820 Druggable Cancer Targets v1.0 library (shRNA) Genetic reagent (Homo sapiens) CP1074 PMID: 27329820 Druggable Cancer Targets v1.0 library (CRISPR-Cas9) Cell line (Homo sapiens) CLF_PEDS0005_N this paper normal kidney cell line Cell line (Homo sapiens) CLF_PEDS0005_T1 this paper primary RMC cell line Cell line (Homo sapiens) CLF_PEDS0005_T2A this paper metastatic RMC cell line (adherent) Cell line (Homo sapiens) CLF_PEDS0005_T2B this paper metastatic RMC cell line (suspension) Cell line (Homo sapiens) CLF_PEDS9001_T1 this paper primary RMC cell line Cell line (Homo sapiens) CLF_PEDS1012_T1 this paper Wilms tumor cell line Cell line (Homo sapiens) G-401 ATCC CRL-1441 RRID:CVCL_0270 Cell line (Homo sapiens) RPMI 8226 ATCC CCL-155 RRID:CVCL_0014 Cell line (Homo sapiens) NCI-H2172 ATCC CRL-5930 RRID:CVCL_1537 Cell line (Homo sapiens) A549 ATCC CCL-185 RRID:CVCL_0023 Cell line (Homo sapiens) HA1E other Cell line maintained in W. C. Hahn lab Cell line (Homo sapiens) JMSU1 PMID: 30777879 RRID:CVCL_2081 Antibody anti-cytokeratin CAM5.2 BD Biosciences 349205 RRID:AB_2134314 Immunohistochemistry Continued on next page Hong et al. eLife 2019;8:e44161. .. DOI: https://doi.org/10.7554/eLife.44161 19 of 32 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Rneasy Plus Mini Kit Qiagen 74134 Commercial assay or kit Qubit RNA HS Assay Kit Thermo Fisher Scientific Q32852 Commercial assay or kit KAPA Stranded mRNA-Seq Kit Kapa Biosystems KK8420 Commercial assay or kit KAPA Library Quantification Kit Kapa Biosystems KK4835 Commercial assay or kit High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific 4368814 Commercial assay or kit Power SYBR Green PCR Master Mix Thermo Fisher Scientific 4368708 Commercial assay or kit Senescence b-Galactosidase Staining Kit Cell Signaling Technology 9860S Commercial assay or kit CellTiter-Glo Luminescent Cell Viability Assay Promega G7570 Commercial assay or kit Proteasome-Glo Chymotrypsin-Like Cell-based Assay Promega G8660 Commercial assay or kit Annexin V: FITC Apoptosis Detection Kit Thermo Fisher Scientific BD 556547 Commercial assay or kit PI/Rnase Staining Buffer BD Pharmingen 550825 Commercial assay or kit FxCycle PI/Rnase Staining Solution Invitrogen F10797 Chemical compound, drug bortezomib (PS-341) Selleck Chemicals S1013 in vitro studies Chemical compound, drug ixazomib (MLN2238) Selleck Chemicals S2180 in vitro studies Chemical compound, drug nocodazole Selleck Chemicals S2775 in vitro studies Chemical compound, drug Ro-3306 Selleck Chemicals S7747 in vitro studies Chemical compound, drug MLN2238 MedChem Express HY-10453 in vivo studies Software, algorithm SvABA v0.2.1 PMID: 29535149 Software, algorithm FlowJo v10.0 RRID:SCR_008520 Software, algorithm ComBat RRID:SCR_010974 Software, algorithm GraphPad Prism v8.0 RRID:SCR_002798 Software, algorithm GSEA Broad Institute RRID:SCR_003199 Software, algorithm GATK v.4.0.4.0 PMID: 20644199 RRID:SCR_001876 Software, algorithm TopHat v2.0.11 PMID: 22383036 RRID:SCR_013035 Software, algorithm DESeq2 PMID: 25516281 RRID:SCR_015687

    Wilms Tumor Assay:

    Article Title: Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.44161 17 of 32 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) UBE2C NA Strain, strain background (Mus musculus) CrTac:NCr-Foxn1nu Taconic Biosciences RRID:IMSR_TAC:ncrnu Genetic reagentLuciferase this paper RRID:Addgene_117072 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) UBE2C guide 1 this paper RRID:Addgene_117068 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) UBE2C guide 2 this paper RRID:Addgene_117071 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) PSMB5 guide 1 this paper RRID:Addgene_117073 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) PSMB5 guide 2 this paper RRID:Addgene_117074 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) pDONR223 SMARCB1 this paper RRID:Addgene_111181 Genetic reagent (Homo sapiens) pLXI_401 LacZ this paper RRID:Addgene_111183 Backbone: pLXI_403 Genetic reagent (Homo sapiens) pLXI_403 LacZ this paper RRID:Addgene_111184 Backbone: pLXI_403 Genetic reagent (Homo sapiens) pLXI_401 SMARCB1 this paper RRID:Addgene_111182 Backbone: pLXI_401 Genetic reagent (Homo sapiens) pLXI_403 SMARCB1 this paper RRID:Addgene_111185 Backbone: pLXI_403 Genetic reagent (Homo sapiens) CP1050 PMID: 27329820 Druggable Cancer Targets v1.0 library (shRNA) Genetic reagent (Homo sapiens) CP1074 PMID: 27329820 Druggable Cancer Targets v1.0 library (CRISPR-Cas9) Cell line (Homo sapiens) CLF_PEDS0005_N this paper normal kidney cell line Cell line (Homo sapiens) CLF_PEDS0005_T1 this paper primary RMC cell line Cell line (Homo sapiens) CLF_PEDS0005_T2A this paper metastatic RMC cell line (adherent) Cell line (Homo sapiens) CLF_PEDS0005_T2B this paper metastatic RMC cell line (suspension) Cell line (Homo sapiens) CLF_PEDS9001_T1 this paper primary RMC cell line Cell line (Homo sapiens) CLF_PEDS1012_T1 this paper Wilms tumor cell line Cell line (Homo sapiens) G-401 ATCC CRL-1441 RRID:CVCL_0270 Cell line (Homo sapiens) RPMI 8226 ATCC CCL-155 RRID:CVCL_0014 Cell line (Homo sapiens) NCI-H2172 ATCC CRL-5930 RRID:CVCL_1537 Cell line (Homo sapiens) A549 ATCC CCL-185 RRID:CVCL_0023 Cell line (Homo sapiens) HA1E other Cell line maintained in W. C. Hahn lab Cell line (Homo sapiens) JMSU1 PMID: 30777879 RRID:CVCL_2081 Antibody anti-cytokeratin CAM5.2 BD Biosciences 349205 RRID:AB_2134314 Immunohistochemistry Continued on next page Hong et al. eLife 2019;8:e44161. .. DOI: https://doi.org/10.7554/eLife.44161 19 of 32 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Rneasy Plus Mini Kit Qiagen 74134 Commercial assay or kit Qubit RNA HS Assay Kit Thermo Fisher Scientific Q32852 Commercial assay or kit KAPA Stranded mRNA-Seq Kit Kapa Biosystems KK8420 Commercial assay or kit KAPA Library Quantification Kit Kapa Biosystems KK4835 Commercial assay or kit High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific 4368814 Commercial assay or kit Power SYBR Green PCR Master Mix Thermo Fisher Scientific 4368708 Commercial assay or kit Senescence b-Galactosidase Staining Kit Cell Signaling Technology 9860S Commercial assay or kit CellTiter-Glo Luminescent Cell Viability Assay Promega G7570 Commercial assay or kit Proteasome-Glo Chymotrypsin-Like Cell-based Assay Promega G8660 Commercial assay or kit Annexin V: FITC Apoptosis Detection Kit Thermo Fisher Scientific BD 556547 Commercial assay or kit PI/Rnase Staining Buffer BD Pharmingen 550825 Commercial assay or kit FxCycle PI/Rnase Staining Solution Invitrogen F10797 Chemical compound, drug bortezomib (PS-341) Selleck Chemicals S1013 in vitro studies Chemical compound, drug ixazomib (MLN2238) Selleck Chemicals S2180 in vitro studies Chemical compound, drug nocodazole Selleck Chemicals S2775 in vitro studies Chemical compound, drug Ro-3306 Selleck Chemicals S7747 in vitro studies Chemical compound, drug MLN2238 MedChem Express HY-10453 in vivo studies Software, algorithm SvABA v0.2.1 PMID: 29535149 Software, algorithm FlowJo v10.0 RRID:SCR_008520 Software, algorithm ComBat RRID:SCR_010974 Software, algorithm GraphPad Prism v8.0 RRID:SCR_002798 Software, algorithm GSEA Broad Institute RRID:SCR_003199 Software, algorithm GATK v.4.0.4.0 PMID: 20644199 RRID:SCR_001876 Software, algorithm TopHat v2.0.11 PMID: 22383036 RRID:SCR_013035 Software, algorithm DESeq2 PMID: 25516281 RRID:SCR_015687

    Immunohistochemistry:

    Article Title: Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.44161 17 of 32 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Gene (Homo sapiens) UBE2C NA Strain, strain background (Mus musculus) CrTac:NCr-Foxn1nu Taconic Biosciences RRID:IMSR_TAC:ncrnu Genetic reagentLuciferase this paper RRID:Addgene_117072 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) UBE2C guide 1 this paper RRID:Addgene_117068 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) UBE2C guide 2 this paper RRID:Addgene_117071 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) PSMB5 guide 1 this paper RRID:Addgene_117073 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) PSMB5 guide 2 this paper RRID:Addgene_117074 Backbone: pXPR_BRD003 Genetic reagent (Homo sapiens) pDONR223 SMARCB1 this paper RRID:Addgene_111181 Genetic reagent (Homo sapiens) pLXI_401 LacZ this paper RRID:Addgene_111183 Backbone: pLXI_403 Genetic reagent (Homo sapiens) pLXI_403 LacZ this paper RRID:Addgene_111184 Backbone: pLXI_403 Genetic reagent (Homo sapiens) pLXI_401 SMARCB1 this paper RRID:Addgene_111182 Backbone: pLXI_401 Genetic reagent (Homo sapiens) pLXI_403 SMARCB1 this paper RRID:Addgene_111185 Backbone: pLXI_403 Genetic reagent (Homo sapiens) CP1050 PMID: 27329820 Druggable Cancer Targets v1.0 library (shRNA) Genetic reagent (Homo sapiens) CP1074 PMID: 27329820 Druggable Cancer Targets v1.0 library (CRISPR-Cas9) Cell line (Homo sapiens) CLF_PEDS0005_N this paper normal kidney cell line Cell line (Homo sapiens) CLF_PEDS0005_T1 this paper primary RMC cell line Cell line (Homo sapiens) CLF_PEDS0005_T2A this paper metastatic RMC cell line (adherent) Cell line (Homo sapiens) CLF_PEDS0005_T2B this paper metastatic RMC cell line (suspension) Cell line (Homo sapiens) CLF_PEDS9001_T1 this paper primary RMC cell line Cell line (Homo sapiens) CLF_PEDS1012_T1 this paper Wilms tumor cell line Cell line (Homo sapiens) G-401 ATCC CRL-1441 RRID:CVCL_0270 Cell line (Homo sapiens) RPMI 8226 ATCC CCL-155 RRID:CVCL_0014 Cell line (Homo sapiens) NCI-H2172 ATCC CRL-5930 RRID:CVCL_1537 Cell line (Homo sapiens) A549 ATCC CCL-185 RRID:CVCL_0023 Cell line (Homo sapiens) HA1E other Cell line maintained in W. C. Hahn lab Cell line (Homo sapiens) JMSU1 PMID: 30777879 RRID:CVCL_2081 Antibody anti-cytokeratin CAM5.2 BD Biosciences 349205 RRID:AB_2134314 Immunohistochemistry Continued on next page Hong et al. eLife 2019;8:e44161. .. DOI: https://doi.org/10.7554/eLife.44161 19 of 32 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Rneasy Plus Mini Kit Qiagen 74134 Commercial assay or kit Qubit RNA HS Assay Kit Thermo Fisher Scientific Q32852 Commercial assay or kit KAPA Stranded mRNA-Seq Kit Kapa Biosystems KK8420 Commercial assay or kit KAPA Library Quantification Kit Kapa Biosystems KK4835 Commercial assay or kit High-Capacity cDNA Reverse Transcription Kit Thermo Fisher Scientific 4368814 Commercial assay or kit Power SYBR Green PCR Master Mix Thermo Fisher Scientific 4368708 Commercial assay or kit Senescence b-Galactosidase Staining Kit Cell Signaling Technology 9860S Commercial assay or kit CellTiter-Glo Luminescent Cell Viability Assay Promega G7570 Commercial assay or kit Proteasome-Glo Chymotrypsin-Like Cell-based Assay Promega G8660 Commercial assay or kit Annexin V: FITC Apoptosis Detection Kit Thermo Fisher Scientific BD 556547 Commercial assay or kit PI/Rnase Staining Buffer BD Pharmingen 550825 Commercial assay or kit FxCycle PI/Rnase Staining Solution Invitrogen F10797 Chemical compound, drug bortezomib (PS-341) Selleck Chemicals S1013 in vitro studies Chemical compound, drug ixazomib (MLN2238) Selleck Chemicals S2180 in vitro studies Chemical compound, drug nocodazole Selleck Chemicals S2775 in vitro studies Chemical compound, drug Ro-3306 Selleck Chemicals S7747 in vitro studies Chemical compound, drug MLN2238 MedChem Express HY-10453 in vivo studies Software, algorithm SvABA v0.2.1 PMID: 29535149 Software, algorithm FlowJo v10.0 RRID:SCR_008520 Software, algorithm ComBat RRID:SCR_010974 Software, algorithm GraphPad Prism v8.0 RRID:SCR_002798 Software, algorithm GSEA Broad Institute RRID:SCR_003199 Software, algorithm GATK v.4.0.4.0 PMID: 20644199 RRID:SCR_001876 Software, algorithm TopHat v2.0.11 PMID: 22383036 RRID:SCR_013035 Software, algorithm DESeq2 PMID: 25516281 RRID:SCR_015687



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